| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.225708 |
| Chromosome: | chromosome 2 |
| Location: | 5664074 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g109683 | (1 of 3) IPR002912//IPR011598 - ACT domain // Myc-type, basic helix-loop-helix (bHLH) domain | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GATCGATAGGCCTTACCAAATACTACTTGC |
| Internal bar code: | GGTTTCCAGACTACGACAGTAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 794 |
| LEAP-Seq percent confirming: | 99.6784 |
| LEAP-Seq n confirming: | 12089 |
| LEAP-Seq n nonconfirming: | 39 |
| LEAP-Seq n unique pos: | 14 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACGCCCAACCCTAAAGAAGT |
| Suggested primer 2: | CTTGTCTCCCGTTTGACCAT |