Insertion junction: LMJ.RY0402.225735_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:+
Strain:LMJ.RY0402.225735
Chromosome:chromosome 9
Location:3189763
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre09.g387356 (1 of 10) PTHR12270//PTHR12270:SF6 - GLYCOSYLTRANSFERASE-RELATED // GLYCOSYLTRANSFERASE-LIKE PROTEIN LARGE 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TGTAATGTACAACACCGCAACGGCAACGTG
Internal bar code:CGGACGGTTGCTTTCAGTTTGA

Confirmation - LEAP-Seq

LEAP-Seq distance:369
LEAP-Seq percent confirming:99.5507
LEAP-Seq n confirming:2659
LEAP-Seq n nonconfirming:12
LEAP-Seq n unique pos:2

Suggested primers for confirmation by PCR

Suggested primer 1:GTGTGCTCCACTCAGGAACA
Suggested primer 2:TGGTGCTCTCTGACACCAAG