Insertion junction: LMJ.RY0402.225767_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.225767
Chromosome:chromosome 13
Location:470592
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre13.g564900 MRP3 (1 of 18) 3.6.3.44 - Xenobiotic-transporting ATPase / Steroid-transporting ATPase; ABC transporter, multidrug resistance associated protein intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TGGGTGTGTGCGTGAGGCGTGCGCCAAGGT
Internal bar code:TCAGGGACACAAGGCGAGAAGA

Confirmation - LEAP-Seq

LEAP-Seq distance:587
LEAP-Seq percent confirming:98.0326
LEAP-Seq n confirming:1445
LEAP-Seq n nonconfirming:29
LEAP-Seq n unique pos:52

Suggested primers for confirmation by PCR

Suggested primer 1:TTGTAGCTCATTTTCGCGTG
Suggested primer 2:CGGATGAGCTGTGTAGACGA