| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.225769 |
| Chromosome: | chromosome 7 |
| Location: | 6021635 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g354750 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCCAGTCTCGTTGCCGACTCCACACCGCTC |
| Internal bar code: | CGCTGGACGTGCCATCGTTAGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 629 |
| LEAP-Seq percent confirming: | 99.4172 |
| LEAP-Seq n confirming: | 3753 |
| LEAP-Seq n nonconfirming: | 22 |
| LEAP-Seq n unique pos: | 52 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GATTGTGTGCCGGAAGAGAT |
| Suggested primer 2: | AGTATTGCTGGTCCCACAGG |