| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.225771 |
| Chromosome: | chromosome 6 |
| Location: | 6338321 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g291950 | MRS4,MRS3 | Mg2+ transporter protein; (1 of 3) K16075 - magnesium transporter (MRS2, MFM1) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTCACGACCAGCGCCCGCTCCCGGATGAGC |
| Internal bar code: | TACTCTGGCCAAATAATTCGGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 352 |
| LEAP-Seq percent confirming: | 98.1047 |
| LEAP-Seq n confirming: | 4762 |
| LEAP-Seq n nonconfirming: | 92 |
| LEAP-Seq n unique pos: | 10 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGAGGGAGGAGCTGTGTTGA |
| Suggested primer 2: | AGGGGGTGCTGTACACTCAG |