Insertion junction: LMJ.RY0402.225778_2

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.225778
Chromosome:chromosome 3
Location:1379978
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre03.g150800 (1 of 45) IPR029052 - Metallo-dependent phosphatase-like CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GCGGCAGCGATATCGTCGCTGCGGTGTCGG
Internal bar code:GTCGTCGGGATGGACCCGGTAG

Confirmation - LEAP-Seq

LEAP-Seq distance:536
LEAP-Seq percent confirming:83.0218
LEAP-Seq n confirming:1066
LEAP-Seq n nonconfirming:218
LEAP-Seq n unique pos:32

Suggested primers for confirmation by PCR

Suggested primer 1:ATGTGTACAGCCGTATGCCA
Suggested primer 2:CGGTGTGTGTGTGTGTGTGT