Insertion cassette: | CIB1 |
Side of cassette: | 5' |
Strand: | + |
Strain: | LMJ.RY0402.225792 |
Chromosome: | chromosome_5 |
Location: | 3267247 |
Confidence (%): | 73 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre05.g240225 | sense | intron |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | CAAGTATGTAAGTAAGGAACAACGCGCAAG |
Internal bar code: | CATGACCCAGTTACATCGAACC |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 699 |
LEAP-Seq percent confirming: | 77.8822 |
LEAP-Seq n confirming: | 9363 |
LEAP-Seq n nonconfirming: | 2659 |
LEAP-Seq n unique pos: | 10 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | TGAACGGACGTGTATTTCCA |
Suggested primer 2: | GGCAGTAGCAGTGAGGGAAG |