Insertion junction: LMJ.RY0402.225845_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.225845
Chromosome:chromosome 3
Location:4306764
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre03.g174750 CYN26,CYN26B,CYN26-2 (1 of 1) PTHR11071//PTHR11071:SF244 - PEPTIDYL-PROLYL CIS-TRANS ISOMERASE // CYCLOPHILIN-LIKE PEPTIDYL-PROLYL CIS-TRANS ISOMERASE FAMILY PROTEIN; Cyclophilin 26-2 CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CGGCGGGGGCCGGGGGGCCTCCGGCCGGGG
Internal bar code:CATACGTGGCGGCCAACGCCTA

Confirmation - LEAP-Seq

LEAP-Seq distance:564
LEAP-Seq percent confirming:97.327
LEAP-Seq n confirming:619
LEAP-Seq n nonconfirming:17
LEAP-Seq n unique pos:25

Suggested primers for confirmation by PCR

Suggested primer 1:TTACCCAAACCCTTCTGCAC
Suggested primer 2:CTGGAGCTGTCATAGAGGGC