Insertion junction: LMJ.RY0402.225845_2

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:+
Strain:LMJ.RY0402.225845
Chromosome:chromosome 3
Location:4306767
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre03.g174750 CYN26,CYN26B,CYN26-2 (1 of 1) PTHR11071//PTHR11071:SF244 - PEPTIDYL-PROLYL CIS-TRANS ISOMERASE // CYCLOPHILIN-LIKE PEPTIDYL-PROLYL CIS-TRANS ISOMERASE FAMILY PROTEIN; Cyclophilin 26-2 CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCGACCGCCAACATGGACCGCCGCGGAGTG
Internal bar code:TACATGCCAATTTGACAAATGA

Confirmation - LEAP-Seq

LEAP-Seq distance:584
LEAP-Seq percent confirming:99.6623
LEAP-Seq n confirming:9148
LEAP-Seq n nonconfirming:31
LEAP-Seq n unique pos:5

Suggested primers for confirmation by PCR

Suggested primer 1:TTACCCAAACCCTTCTGCAC
Suggested primer 2:CTGGAGCTGTCATAGAGGGC