| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.225847 |
| Chromosome: | chromosome 14 |
| Location: | 406039 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre14.g610631 | (1 of 25) IPR000210//IPR011333 - BTB/POZ domain // POZ domain | 3'UTR_intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTTATACGGTAGCGCCCCTGTGCGGCCCAC |
| Internal bar code: | TATGCAGTCCGTCGGAATTTCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 46 |
| LEAP-Seq percent confirming: | 77.0492 |
| LEAP-Seq n confirming: | 47 |
| LEAP-Seq n nonconfirming: | 14 |
| LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTGCATGCTACATCGTTCGT |
| Suggested primer 2: | CTGGACCAGACCAACACCTT |