Insertion junction: LMJ.RY0402.225875_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.225875
Chromosome:chromosome 2
Location:7516717
Confidence (%):58
Locus systematic id Locus common name Defline Feature
Cre02.g144005 (1 of 4) 2.3.1.43 - Phosphatidylcholine--sterol O-acyltransferase / Phospholipid--cholesterol acyltransferase intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCAACCCCCCCTACGTGGCAAGCAAAGCAC
Internal bar code:CTGGACACGCAACTTTTGCAAG

Confirmation - LEAP-Seq

LEAP-Seq distance:137
LEAP-Seq percent confirming:78.0347
LEAP-Seq n confirming:135
LEAP-Seq n nonconfirming:38
LEAP-Seq n unique pos:6

Suggested primers for confirmation by PCR

Suggested primer 1:CACCTCAGCAGCCTACACAA
Suggested primer 2:AGTGCCTACCCTTCCCTTGT