| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.225885 |
| Chromosome: | chromosome 7 |
| Location: | 4266579 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g341700 | (1 of 1) K08866 - serine/threonine-protein kinase TTK/MPS1 (TTK, MPS1) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATCGCAGTGTAAGCTAGGGCCTGTGCCCAG |
| Internal bar code: | ATTGAAATCAGGCGGTTATTAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 539 |
| LEAP-Seq percent confirming: | 98.9637 |
| LEAP-Seq n confirming: | 955 |
| LEAP-Seq n nonconfirming: | 10 |
| LEAP-Seq n unique pos: | 17 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGTCTGCTCTTTCCACATGC |
| Suggested primer 2: | TGTTGGTGCAGTGATTGGTT |