Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | + |
Strain: | LMJ.RY0402.225906 |
Chromosome: | chromosome_16 |
Location: | 1941698 |
Confidence (%): | 73 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre16.g656300 | DPR1 | Dihydrodipicolinate reductase | sense | CDS |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | ATCGGCGTGCGCGGCGTGGCCACCCAGCTG |
Internal bar code: | GGGCCGAGCGCATACCAGATAC |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 654 |
LEAP-Seq percent confirming: | 99.562 |
LEAP-Seq n confirming: | 1591 |
LEAP-Seq n nonconfirming: | 7 |
LEAP-Seq n unique pos: | 20 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | AAGATACGATGGCAACCTCG |
Suggested primer 2: | CTTGCTGGTAGGAGGACTGC |