Insertion junction: LMJ.RY0402.225906_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.225906
Chromosome:chromosome_16
Location:1941698
Confidence (%):73
Locus systematic id Locus common name Defline Orientation Feature
Cre16.g656300 DPR1 Dihydrodipicolinate reductase sense CDS

Insertion site details

Flanking sequence (orientation from cassette outwards):ATCGGCGTGCGCGGCGTGGCCACCCAGCTG
Internal bar code:GGGCCGAGCGCATACCAGATAC

Confirmation - LEAP-Seq

LEAP-Seq distance:654
LEAP-Seq percent confirming:99.562
LEAP-Seq n confirming:1591
LEAP-Seq n nonconfirming:7
LEAP-Seq n unique pos:20

Suggested primers for confirmation by PCR

Suggested primer 1:AAGATACGATGGCAACCTCG
Suggested primer 2:CTTGCTGGTAGGAGGACTGC