| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.225921 |
| Chromosome: | chromosome 2 |
| Location: | 703829 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g078300 | GPX1,PHGPx1 | Glutathione peroxidase 1, selenoprotein; (1 of 4) 1.11.1.9 - Glutathione peroxidase | 5'UTR_intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGAAAGAACGTCTCAAGAGAGATACCGAGG |
| Internal bar code: | TCGCTCGTGACTTGAGACAGCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 608 |
| LEAP-Seq percent confirming: | 97.561 |
| LEAP-Seq n confirming: | 680 |
| LEAP-Seq n nonconfirming: | 17 |
| LEAP-Seq n unique pos: | 16 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CACAATCACTGTCCGAGGTG |
| Suggested primer 2: | TGATATGAACGGTTGGGGAT |