Insertion junction: LMJ.RY0402.225930_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.225930
Chromosome:chromosome_8
Location:2522102
Confidence (%):58
Locus systematic id Locus common name Defline Orientation Feature
None

Insertion site details

Flanking sequence (orientation from cassette outwards):CCCCGCCGTCACGCAGACATATGTAATTTG
Internal bar code:ACTCGTGTAGCGAATTCACGCC

Confirmation - LEAP-Seq

LEAP-Seq distance:685
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:2
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:2

Suggested primers for confirmation by PCR

Suggested primer 1:ACACCAGACCAGACCAGACC
Suggested primer 2:AGCGGTCTCCTTCATCTTGA