| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.225944 |
| Chromosome: | chromosome 6 |
| Location: | 7788667 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g302400 | TNP17,CSB31 | {"Probable transposon-derived protein of Chlamydomonas-Specific family B; (1 of 1) IPR000104//IPR010095 - Antifreeze protein, type I // Transposase IS605, OrfB, C-terminal | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGGAAACTCAGTCAAAACTCGAAGGAAACT |
| Internal bar code: | TTGCCTTAAGAAATTGAGGATG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 502 |
| LEAP-Seq percent confirming: | 77.7778 |
| LEAP-Seq n confirming: | 42 |
| LEAP-Seq n nonconfirming: | 12 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGTAGCTGTACGGCTGGAAG |
| Suggested primer 2: | ACAGCCCGTGTCAGGAATAC |