| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.225947 |
| Chromosome: | chromosome 17 |
| Location: | 3357325 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g723500 | (1 of 1) 3.6.3.30 - Fe(3+)-transporting ATPase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGTTGGAGCGCGGGGTTGGGGCGCGGGGTG |
| Internal bar code: | CATCCTTCGGGTCGGAATTGGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 909 |
| LEAP-Seq percent confirming: | 98.8439 |
| LEAP-Seq n confirming: | 171 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCCTCCCACCTAAACTCCTC |
| Suggested primer 2: | GATTCCATGTTGTGTTCCCC |