| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.225950 |
| Chromosome: | chromosome 1 |
| Location: | 797405 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g004124 | (1 of 3) 3.2.1.8 - Endo-1,4-beta-xylanase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTCCTGATGCTCCGGGCTCAGCCCATTTCC |
| Internal bar code: | GGGCACGGGACTGTTTTCCGGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 750 |
| LEAP-Seq percent confirming: | 97.8549 |
| LEAP-Seq n confirming: | 11906 |
| LEAP-Seq n nonconfirming: | 261 |
| LEAP-Seq n unique pos: | 112 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTGCTTGCAAGTGCTGTCTC |
| Suggested primer 2: | CCGTGTGTGTGTGTGTGTGT |