| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.225962 |
| Chromosome: | chromosome 12 |
| Location: | 290149 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g485550 | (1 of 1) 4.1.3.4 - Hydroxymethylglutaryl-CoA lyase / HMG-CoA lyase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTGCGCCCACAAATGTTGGTCACGCTCTAT |
| Internal bar code: | GCGTCGCAGCTGAGACGCGAGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 271 |
| LEAP-Seq percent confirming: | 99.0964 |
| LEAP-Seq n confirming: | 329 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 12 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GACGGCATATCAATGCACAC |
| Suggested primer 2: | CCAGCCCAAACTAAACCAAA |