Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | - |
Strain: | LMJ.RY0402.225984 |
Chromosome: | chromosome_9 |
Location: | 3233236 |
Confidence (%): | 58 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre09.g387689 | FAP76 | Flagellar Associated Protein | antisense | intron |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | TGCGGTCTCTCGCCCCCCTCGCGCGCTGTC |
Internal bar code: | AGAGTGCTAAAGAGCGACATGG |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 535 |
LEAP-Seq percent confirming: | 99.2462 |
LEAP-Seq n confirming: | 395 |
LEAP-Seq n nonconfirming: | 3 |
LEAP-Seq n unique pos: | 20 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | GAATAAACTGCCCCACTCCA |
Suggested primer 2: | GTAACCTGCACACGTCCCTT |