| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.225985 |
| Chromosome: | chromosome_3 |
| Location: | 7644133 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre03.g204150 | FAP167,IFT80 | Intraflagellar transport protein 80 | antisense | CDS |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | GTAGCGCTTGGCGCCTGGCCGCTGCGACTC |
| Internal bar code: | AAGGAGCCATCGAGCTCGGCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 604 |
| LEAP-Seq percent confirming: | 75.2 |
| LEAP-Seq n confirming: | 564 |
| LEAP-Seq n nonconfirming: | 186 |
| LEAP-Seq n unique pos: | 16 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACAGAAGCGGCATCAGAGTT |
| Suggested primer 2: | GCCATCAAGCTGAACATCAA |