Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | + |
Strain: | LMJ.RY0402.226067 |
Chromosome: | chromosome_4 |
Location: | 1999172 |
Confidence (%): | 73 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre04.g218800 | THB3 | Truncated hemoglobin | antisense | 3'UTR |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | TTTGCAAGGATTACACTGATTTAAACTCTG |
Internal bar code: | CGGTTGGCGTTTTATCCCGCAT |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 607 |
LEAP-Seq percent confirming: | 96.5079 |
LEAP-Seq n confirming: | 304 |
LEAP-Seq n nonconfirming: | 11 |
LEAP-Seq n unique pos: | 14 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | AGGTGTCAACCGACTGCTCT |
Suggested primer 2: | GCTACGCTTCAGAATTTGCC |