| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.226071 |
| Chromosome: | chromosome_17 |
| Location: | 1089444 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre17.g703900 | IFT172 | Intraflagellar transport protein 172 | sense | intron |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | CTCTCTCCTTGTGCTACTGCATCCCACCCG |
| Internal bar code: | GGTGTAACTCGATACTCGGCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 684 |
| LEAP-Seq percent confirming: | 99.515 |
| LEAP-Seq n confirming: | 2052 |
| LEAP-Seq n nonconfirming: | 10 |
| LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAGAACTACTACCTGGCCGC |
| Suggested primer 2: | CCGCTCTACGGTGCTTCTAC |