| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.226073 |
| Chromosome: | chromosome 9 |
| Location: | 6552074 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g407950 | (1 of 2) IPR004911//IPR012336 - Gamma interferon inducible lysosomal thiol reductase GILT // Thioredoxin-like fold | 5'UTR_intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCCAAGTTGCAGTATGATAGACCGTTGTGG |
| Internal bar code: | TACCTGAGGGCCAAGTGATACT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 73 |
| LEAP-Seq percent confirming: | 77.7778 |
| LEAP-Seq n confirming: | 7 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACGGTTACTATGGTGCGGAG |
| Suggested primer 2: | AAGCATAGTGCAAACCCACC |