| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.226087 |
| Chromosome: | chromosome 3 |
| Location: | 1730972 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g153600 | (1 of 1) IPR000104//IPR003034//IPR022656 - Antifreeze protein, type I // SAP domain // XPA C- terminal | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCAAGCGTGTGCGTGCGTGTGTGTGCAGCC |
| Internal bar code: | TTGAGAGTACATCTTCTGACAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 747 |
| LEAP-Seq percent confirming: | 99.3652 |
| LEAP-Seq n confirming: | 22070 |
| LEAP-Seq n nonconfirming: | 141 |
| LEAP-Seq n unique pos: | 18 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAAACCAAACCAAGCCAACT |
| Suggested primer 2: | AGGAAGAGGAGGAGGCAGAG |