| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.226094 |
| Chromosome: | chromosome 7 |
| Location: | 2617808 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g330300 | (1 of 2) K14411 - RNA-binding protein Musashi (MSI) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTTACCCACGTCTTATCGACATAGGATATC |
| Internal bar code: | GAGGTTTAGTACGGTGTTCTTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 415 |
| LEAP-Seq percent confirming: | 99.3411 |
| LEAP-Seq n confirming: | 2563 |
| LEAP-Seq n nonconfirming: | 17 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATCCTGTCGTTGGTAGTGGC |
| Suggested primer 2: | ATAACCTTAACTGGGCGGCT |