| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.226103 |
| Chromosome: | chromosome 8 |
| Location: | 3546961 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre08.g375450 | SDR13 | (1 of 1) 1.1.1.243 - Carveol dehydrogenase; Short-chain dehydrogenase/reductase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCCAAAACAGCACCCAGCAAACGCATTTTC |
| Internal bar code: | TGGTCGGCTACGCACCCTGTAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 635 |
| LEAP-Seq percent confirming: | 99.5951 |
| LEAP-Seq n confirming: | 738 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 24 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AACTGATTTGGGTCCAGTCG |
| Suggested primer 2: | TGTGCTTGTGGGAGAGAGTG |