| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.226120 |
| Chromosome: | chromosome 6 |
| Location: | 6733118 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g294450 | AKR6 | Aldo/keto reductase; (1 of 1) PTHR11732//PTHR11732:SF150 - ALDO/KETO REDUCTASE // SUBFAMILY NOT NAMED | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AAGGCTCAGTGCTCGACGCCACGCGCCCAT |
| Internal bar code: | TGTCGGCTCTGAAAGGCAAGCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 581 |
| LEAP-Seq percent confirming: | 98.0233 |
| LEAP-Seq n confirming: | 843 |
| LEAP-Seq n nonconfirming: | 17 |
| LEAP-Seq n unique pos: | 20 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TAGTGGTTGTGGAGCTGTGG |
| Suggested primer 2: | CCCCTTATTCGGACCAATTT |