Insertion junction: LMJ.RY0402.226127_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.226127
Chromosome:chromosome 5
Location:337339
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre05.g233303 (1 of 1) K11718 - UDP-glucose:glycoprotein glucosyltransferase (HUGT) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ATGATCTTGTCTCAACTACTCATGCATGGA
Internal bar code:CAATCGTTCCACATTTAGGCAC

Confirmation - LEAP-Seq

LEAP-Seq distance:1039
LEAP-Seq percent confirming:93.4619
LEAP-Seq n confirming:2230
LEAP-Seq n nonconfirming:156
LEAP-Seq n unique pos:86

Suggested primers for confirmation by PCR

Suggested primer 1:ACCCGTGCTGAACAAAAATC
Suggested primer 2:AAAAAGAGAGACGGGTGCCT