Insertion junction: LMJ.RY0402.226139_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.226139
Chromosome:chromosome 14
Location:1989373
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre14.g621400 (1 of 1) IPR001810//IPR025886 - F-box domain // Phloem protein 2-like intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GTTGGTGTCACAGGTCACCAGCTGATTGTC
Internal bar code:GCGCTAAAGTGATTCGGCTTGG

Confirmation - LEAP-Seq

LEAP-Seq distance:599
LEAP-Seq percent confirming:88.6146
LEAP-Seq n confirming:1759
LEAP-Seq n nonconfirming:226
LEAP-Seq n unique pos:39

Suggested primers for confirmation by PCR

Suggested primer 1:GGACGAGACGAACTAGGTGC
Suggested primer 2:AGGCGTCCCAGATCTGTCTA