| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.226158 |
| Chromosome: | chromosome_17 |
| Location: | 514635 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre17.g699500 | TTL8,TTLL9,FAP267 | Tubulin tyrosine ligase | sense | CDS |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | TCTGTTTCACTCTCCGTCTCCACCCATTCA |
| Internal bar code: | TTTAGGAGAGGTGAGTCGTCGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 436 |
| LEAP-Seq percent confirming: | 98.6906 |
| LEAP-Seq n confirming: | 13115 |
| LEAP-Seq n nonconfirming: | 174 |
| LEAP-Seq n unique pos: | 14 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAAGAAAGCCGCTGGAATAG |
| Suggested primer 2: | ATCGTGGTTTACCGAGATGC |