| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.226172 |
| Chromosome: | chromosome 10 |
| Location: | 3999076 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g448950 | EEP3 | Exonuclease-Endonuclease-Phosphatase superfamily protein; (1 of 1) K18764 - nocturnin [EC:3.1.13.4] (CCRN4L) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGGACTCCAAAGAGCCCAAGCGTGGCAGCA |
| Internal bar code: | CATGGGCGCAGTGCGCGGACTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 565 |
| LEAP-Seq percent confirming: | 99.6396 |
| LEAP-Seq n confirming: | 553 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 19 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCAGTCTTGCACAAGCCATA |
| Suggested primer 2: | TTGCTGCTCTGTATGTTGGC |