| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.226173 |
| Chromosome: | chromosome 11 |
| Location: | 3045762 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre11.g478800 | (1 of 1) PTHR31027//PTHR31027:SF2 - FAMILY NOT NAMED // PROTEIN F18H3.4 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CACCCTTCGCTGCTCCCCTCCAAAACAACC |
| Internal bar code: | GCGATTGGCGGGGAGGGGTGCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 585 |
| LEAP-Seq percent confirming: | 99.3976 |
| LEAP-Seq n confirming: | 165 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 12 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATGGCTGAAAATTTTGGTCG |
| Suggested primer 2: | ACCATGCAAGTTTGGTGTCA |