| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.226184 |
| Chromosome: | chromosome 17 |
| Location: | 4055734 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g729200 | (1 of 1) PF04678 - Mitochondrial calcium uniporter (MCU) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCGAGGCTTCGTGGCTCTGAGTGCTGTTCC |
| Internal bar code: | CGACCCTAAGAGGCTGGCGTAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 228 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 649 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCACTGCCACAGTAGCCAAG |
| Suggested primer 2: | CCAGAGGTGGGCATAGGTAA |