| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.226188 |
| Chromosome: | chromosome 2 |
| Location: | 951344 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g079850 | POLK1 | (1 of 1) K03511 - DNA polymerase kappa (POLK); DNA polymerase kappa | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTGGAGCTGGCCCCCACCTGCTTGGCCATG |
| Internal bar code: | TTCGGGTATCGACGTGTCCCAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 263 |
| LEAP-Seq percent confirming: | 99.7926 |
| LEAP-Seq n confirming: | 4331 |
| LEAP-Seq n nonconfirming: | 9 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TATCTCTCATACCCGGCACC |
| Suggested primer 2: | CATCTCAGGCTTCAGTGCAA |