Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | + |
Strain: | LMJ.RY0402.226211 |
Chromosome: | chromosome 12 |
Location: | 9526370 |
Confidence (%): | 73 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
Cre12.g543902 | (1 of 1) 2.7.1.16 - Ribulokinase / L-ribulokinase | intron |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATTCCTAGCTTGCTGCTGCACGCCGCCCTG |
Internal bar code: | ACGGAACTGCGCGGCGAGATTA |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 963 |
LEAP-Seq percent confirming: | 76.1463 |
LEAP-Seq n confirming: | 5580 |
LEAP-Seq n nonconfirming: | 1748 |
LEAP-Seq n unique pos: | 58 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | ACGGTCCATGATATCCCAAA |
Suggested primer 2: | AGAGTCCTAGCCCTGAAGCC |