| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.226214 |
| Chromosome: | chromosome 5 |
| Location: | 636290 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre05.g232004 | (1 of 1) IPR012677//IPR014720 - Nucleotide-binding alpha-beta plait domain // Double-stranded RNA-binding domain | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATGCAAGCGTGCCAAGCCCTGTAGCCCGAG |
| Internal bar code: | GCACTTACTTATAGTACACTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 362 |
| LEAP-Seq percent confirming: | 77.7614 |
| LEAP-Seq n confirming: | 7913 |
| LEAP-Seq n nonconfirming: | 2263 |
| LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACTGCATCTAAGCCCACACC |
| Suggested primer 2: | CCTCTCAGACCAACCTGAGC |