| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.226214 |
| Chromosome: | chromosome 5 |
| Location: | 2641411 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre05.g236450 | 5'UTR_intron|intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCGACACCAGCTGTCGCAAACTCACCAGCT |
| Internal bar code: | TAAGAGGTTCGCGTGTGGTATT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 611 |
| LEAP-Seq percent confirming: | 92.1596 |
| LEAP-Seq n confirming: | 4643 |
| LEAP-Seq n nonconfirming: | 395 |
| LEAP-Seq n unique pos: | 59 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCCTTGAGAAAGACCACAGC |
| Suggested primer 2: | GTGCACATGCGGTACATTTC |