| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.226217 |
| Chromosome: | chromosome 6 |
| Location: | 8764855 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g309951 | (1 of 1) 3.2.1.18 - Exo-alpha-sialidase / Sialidase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACTTGCAGGCCGGTGGTGAACTCCAGCGTG |
| Internal bar code: | TTGGCTGTACGTCAGCGTCGAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 741 |
| LEAP-Seq percent confirming: | 98.3553 |
| LEAP-Seq n confirming: | 4186 |
| LEAP-Seq n nonconfirming: | 70 |
| LEAP-Seq n unique pos: | 57 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGGTCCTCCTCCTCGTAGTC |
| Suggested primer 2: | GACACGTGGGATGTATGCTG |