Insertion junction: LMJ.RY0402.226225_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.226225
Chromosome:chromosome 17
Location:2437465
Confidence (%):58
Locus systematic id Locus common name Defline Feature
Cre17.g715700 VTC3,PPP44,PP2C5 (1 of 1) PF00481//PF07714 - Protein phosphatase 2C (PP2C) // Protein tyrosine kinase (Pkinase_Tyr); protein phosphatase 2C-like intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCTGAATTTGGTTTCGACTGAACCCCACCT
Internal bar code:GCTCAATTCCCTTGCCCAGGAT

Confirmation - LEAP-Seq

LEAP-Seq distance:281
LEAP-Seq percent confirming:98.0
LEAP-Seq n confirming:196
LEAP-Seq n nonconfirming:4
LEAP-Seq n unique pos:8

Suggested primers for confirmation by PCR

Suggested primer 1:AACAGGACCAACTGCAAACC
Suggested primer 2:GAGTACTTCACGCACTGGCA