| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.226227 |
| Chromosome: | chromosome 2 |
| Location: | 4815250 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g102900 | (1 of 55) PTHR23257//PTHR23257:SF394 - SERINE-THREONINE PROTEIN KINASE // SUBFAMILY NOT NAMED | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATGACCTCGCTGTGCTCCGCTGCGTCGTGA |
| Internal bar code: | CCCCTACCAGTGCTTCGGGCAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 561 |
| LEAP-Seq percent confirming: | 97.9222 |
| LEAP-Seq n confirming: | 1461 |
| LEAP-Seq n nonconfirming: | 31 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTGTCCATTGCTTGAAGCTG |
| Suggested primer 2: | CATCCCCAACTTTACCTCCA |