| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.226249 |
| Chromosome: | chromosome 16 |
| Location: | 2178076 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g658250 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCCTGTTGGTGCCGGTGCTGCTGGTCGGAG |
| Internal bar code: | CCGGGTGGTCCCCCGGGGCTTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 918 |
| LEAP-Seq percent confirming: | 98.9974 |
| LEAP-Seq n confirming: | 15008 |
| LEAP-Seq n nonconfirming: | 152 |
| LEAP-Seq n unique pos: | 25 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CACTCCTTGGCAGTCTCACA |
| Suggested primer 2: | AGTCTGACACTACTGCCGGG |