| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.226265 |
| Chromosome: | chromosome 9 |
| Location: | 3925799 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g392282 | DHC2 | Axonemal Dynein Heavy Chain 2; (1 of 1) PTHR10676:SF137 - DYNEIN HEAVY CHAIN 1, AXONEMAL | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGGATCAACCGCGGCGTGGCGCTGTTCACT |
| Internal bar code: | TCGCTACTCGTTGGTCTGTATC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 483 |
| LEAP-Seq percent confirming: | 98.4615 |
| LEAP-Seq n confirming: | 1344 |
| LEAP-Seq n nonconfirming: | 21 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AATGCCTTAGTCATCGTCCG |
| Suggested primer 2: | TACTGGATCAGCGGCTTCTT |