| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.226285 |
| Chromosome: | chromosome 12 |
| Location: | 9550696 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g546027 | (1 of 2) PF13472 - GDSL-like Lipase/Acylhydrolase family (Lipase_GDSL_2) | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CAAAGATGGTTCAATGCAGGACGACGAGTT |
| Internal bar code: | GCTAAGGACAAGACTCGCGGTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 748 |
| LEAP-Seq percent confirming: | 95.4485 |
| LEAP-Seq n confirming: | 713 |
| LEAP-Seq n nonconfirming: | 34 |
| LEAP-Seq n unique pos: | 23 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGCAAGCAGTGATCTTGTCA |
| Suggested primer 2: | TTACCGCCAGCGCTACTACT |