| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.226290 |
| Chromosome: | chromosome 17 |
| Location: | 3700315 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g726983 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGAGCAGATTGGGCCGGAGCCCGTGCTCGT |
| Internal bar code: | GGCAACATAAGGACGGGAGTGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 767 |
| LEAP-Seq percent confirming: | 99.3494 |
| LEAP-Seq n confirming: | 1527 |
| LEAP-Seq n nonconfirming: | 10 |
| LEAP-Seq n unique pos: | 29 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGAGATGATGAATCGCTGGT |
| Suggested primer 2: | CTTGCTCTTCCACTTCCCAG |