Insertion junction: LMJ.RY0402.226323_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:+
Strain:LMJ.RY0402.226323
Chromosome:chromosome 7
Location:2203502
Confidence (%):58
Locus systematic id Locus common name Defline Feature
Cre07.g327100 (1 of 12) IPR000326 - Phosphatidic acid phosphatase type 2/haloperoxidase intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): AGAATCCGCCGTGGTTGACACGGCCTTGCC
Internal bar code:TTGTACTACATTAACTGGCGGC

Confirmation - LEAP-Seq

LEAP-Seq distance:593
LEAP-Seq percent confirming:99.7537
LEAP-Seq n confirming:405
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:6

Suggested primers for confirmation by PCR

Suggested primer 1:ACTTGGTTTGGTTTCGTTCG
Suggested primer 2:CTCAACGTCGAGCAGGTACA