| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.226335 |
| Chromosome: | chromosome 3 |
| Location: | 7509518 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g205350 | EXN8 | (1 of 7) IPR002562//IPR012337 - 3'-5' exonuclease domain // Ribonuclease H-like domain; 3'-5' exonuclease | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCGATGGCACGCTGAGCGAGCGGCAGTTCG |
| Internal bar code: | CAAGGCTTTCGGCGCGCAGGGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 360 |
| LEAP-Seq percent confirming: | 99.1446 |
| LEAP-Seq n confirming: | 1159 |
| LEAP-Seq n nonconfirming: | 10 |
| LEAP-Seq n unique pos: | 16 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTGATGCCACAGGTCTGCTA |
| Suggested primer 2: | AGCTACCTGTGCGGTCAGTC |