| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.226348 |
| Chromosome: | chromosome_8 |
| Location: | 3832372 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre08.g377500 | antisense | intron |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | TAAACTACGTACCCCCACCCCCCCCCACAC |
| Internal bar code: | TCTCTCTGGAGTAGGTAAGATC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 279 |
| LEAP-Seq percent confirming: | 69.4046 |
| LEAP-Seq n confirming: | 1259 |
| LEAP-Seq n nonconfirming: | 555 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGGGAGAGGGAGAGAATTTG |
| Suggested primer 2: | ACACACAAAGCGCACAGAAC |