| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.226361 |
| Chromosome: | chromosome 5 |
| Location: | 184641 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre05.g234350 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CATGAATGTAACCCCCGTCCAGCCAAAGCA |
| Internal bar code: | ATCGGCGATGGCACTGGATGGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 612 |
| LEAP-Seq percent confirming: | 99.2576 |
| LEAP-Seq n confirming: | 2273 |
| LEAP-Seq n nonconfirming: | 17 |
| LEAP-Seq n unique pos: | 39 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATGGTCACGTTGACGTTTCA |
| Suggested primer 2: | GTTGCCTTTAGGGCATGTGT |