| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.226384 |
| Chromosome: | chromosome 16 |
| Location: | 1625874 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g654250 | THB6 | Truncated hemoglobin; (1 of 2) PTHR10681:SF17 - PEROXIREDOXIN-6 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGAGCGCAGGAGATGCGTACTCTCCTGGGC |
| Internal bar code: | CGACCCGTTGAACTGGTTATGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 568 |
| LEAP-Seq percent confirming: | 98.8203 |
| LEAP-Seq n confirming: | 1424 |
| LEAP-Seq n nonconfirming: | 17 |
| LEAP-Seq n unique pos: | 27 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCATGCCACTCGTAGTGTGT |
| Suggested primer 2: | CTGAGGACCTGCTAGGTTGC |